The Effect of Excess Iron on the Impairment of Glucose Metabolism in Mice
Keywords:
Excess Iron, Glucose Metabolism Disorder, Intraperitoneal Glucose Tolerance Test (IPGTT), Intaperitoneal Pyruvate Tolerance Test (IPTT).Abstract
Excess iron in the body can trigger pathological conditions through production of reactive oxygen species (ROS) and the deposition in various organs. This condition can also disrupt whole metabolism process, including glucose metabolism. However, the mechanisms between iron and glucose metabolism remain unclear. The study was to investigate the effect of excess iron with glucose metabolism disorder by assessing Glucose Tolerance Test (GTT) and gluconeogenesis rate in mice through Intraperitoneal Glucose Tolerance Test (IPGTT) to determine insulin resistance, Intraperitoneal Pyruvate Tolerance Test (IPTT) to measure hepatic gluconeogenesis, and to assess pancreatic histology for routine histogical examination. In this experimental study, eight-teen male mice were assigned to three equal groups. Mice were divided by the saline injected group (control) and iron dextran injection 0.1 mg/mice (group 2) and 0.3 mg/mice (group 3) dose of injection. Iron dextran was injected daily intraperitoneally. After 14 days of treatment, IPGTT, IPTT and pancreas histology were examined. Repeated analysis of variance (ANOVA) with bonferroni’s posthoc multiple comparison test were used for data analysis. IPGTT results showed glucose level was lower 39.85 % in group 3 compared to the control group mice. The results of IPTT showed that glucose level in mice treated with iron dextran were significantly lower in dose dependent manner. Pancreas histology showed islet cells in group 3 decreased in size, which might be due to beta cells depletion. Short term iron injection increases glucose tolerance and supresses hepatic gluconeogenesis in mice.References
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Additional Files
- Data Set
- IPGTT result
- IPTT Result
- Histology of Pancreas
- Research instrument
- Body iron metabolism and pathophysiology of iron overload
- Backe M Balslev, Ingrid Wahl Moen, Christina Ellervik, Jakob BH, Thomas M-P. Iron Regulation of Pancreatic Beta-Cell Functions and Oxidative stress. Annu. Rev. Nutr. 2016;3610.1–10.33.
- Fernández-Real JM, McClain D, Manco M. Mechanisms Linking Glucose Homeostasis and Iron Metabolism Toward the Onset and Progression of Type 2 Diabetes. Diabetes Care. 2015;38(11)2169-76
- Kalhan Satish C., Arnab Ghosh. Dietary Iron, Circadian Clock, and Hepatic Gluconeogenesis. Diabetes. 2015;641091–1093.
- Huang J, Simcox J, Mitchell TC, et al. Iron regulates glucose homeostasis in liver and muscle via AMP-activated protein kinase in mice. FASEB J 2013;27 2845–2854.
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