Biocompatibility Assessment of Newly Fabricated Melt Derived 45S5 Bioactive Glass on Dental Cells Proliferation
Abstract
Abstract—The purpose of this study was to fabricate bioactive glass and to assess the bioactive glass (BG) biocompatibility through the assessment of proliferation rate of stem cells from human exfoliated deciduous teeth (SHED) in the presence of five different concentrations of 45S5 bioactive glass conditioned medium (1 to 10 mg/ml powder to liquid ratio) using alamarBlue assay. 45S5 bioactive glass known as Bioglass® in mole percentages of 46.13% SiO2, 26.91% CaO, 24.35% Na2O and 2.60% P2O5 was fabricated using melt-quenching technique and then the glass was characterized by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) confirming the composition and its amorphous state. Stem cells from human exfoliated deciduous tooth (SHED) were used to evaluate the biocompatibility of 45S5 by exposing the cells to various concentration of BG-conditioned medium (1-10 mg/ml) using alamarBlue assay. XRD result indicated that the BG produced has an amorphous structure. FTIR analysis showed the Si-O-Si bending, Si-O stretching and Si-O-Si stretching (asymmetric) bands present within the BG structure indicating the characteristic of silicate network. For alamarBlue assay, SHED exposed to BG-conditioned medium showed increasing proliferative activity at lower concentration of BG powder to liquid ratio from Days 1 to 7. Hence, it can be concluded that the 45S5 BG with lower concentration of powder to liquid ratio promoted SHED proliferation which can be used for future work.References
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